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control wt mef cells  (ATCC)


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    Structured Review

    ATCC control wt mef cells
    <t>OPA1-Exon4b</t> is not required for mitochondrial fusion but ensues mitochondrial bioenergetic recovery. (A) Labeling of one subset of mitochondria by photoactivation of PAGFP in cells expressing both mtPAGFP and mtDsRed, as indicated on the top (scale bar: 10 μm). (B) Quantitation of mitochondrial fusion events, including kiss-and-run and complete fusion, per run ( n = 10 runs). (C) Δψm of WT <t>MEF</t> cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. The mean TMRM FI of mitochondria in WT cells was normalized to 1. The TMRM FI per mitochondrion below 0.3 denoted low (white arrow). (D) Quantitation of percentage of mitochondria with low Δψm in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (≥20 cells for three biological replicates). (E) Quantitation of percentage of mitochondria with low Δψm in Opa1 KO cells expressing ShLuc plus Flag and Opa1 KO cells expressing OPA1-iso5-Flag plus ShLuc, Sh Mfn 1, Sh Mfn 2 or Sh Mfn 1/2 (≥20 cells for three biological replicates). ** p < 0.01, one-way ANOVA.
    Control Wt Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 631 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control wt mef cells/product/ATCC
    Average 97 stars, based on 631 article reviews
    control wt mef cells - by Bioz Stars, 2026-02
    97/100 stars

    Images

    1) Product Images from "OPA1-Exon4b Binds to mtDNA D-Loop for Transcriptional and Metabolic Modulation, Independent of Mitochondrial Fusion"

    Article Title: OPA1-Exon4b Binds to mtDNA D-Loop for Transcriptional and Metabolic Modulation, Independent of Mitochondrial Fusion

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2020.00180

    OPA1-Exon4b is not required for mitochondrial fusion but ensues mitochondrial bioenergetic recovery. (A) Labeling of one subset of mitochondria by photoactivation of PAGFP in cells expressing both mtPAGFP and mtDsRed, as indicated on the top (scale bar: 10 μm). (B) Quantitation of mitochondrial fusion events, including kiss-and-run and complete fusion, per run ( n = 10 runs). (C) Δψm of WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. The mean TMRM FI of mitochondria in WT cells was normalized to 1. The TMRM FI per mitochondrion below 0.3 denoted low (white arrow). (D) Quantitation of percentage of mitochondria with low Δψm in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (≥20 cells for three biological replicates). (E) Quantitation of percentage of mitochondria with low Δψm in Opa1 KO cells expressing ShLuc plus Flag and Opa1 KO cells expressing OPA1-iso5-Flag plus ShLuc, Sh Mfn 1, Sh Mfn 2 or Sh Mfn 1/2 (≥20 cells for three biological replicates). ** p < 0.01, one-way ANOVA.
    Figure Legend Snippet: OPA1-Exon4b is not required for mitochondrial fusion but ensues mitochondrial bioenergetic recovery. (A) Labeling of one subset of mitochondria by photoactivation of PAGFP in cells expressing both mtPAGFP and mtDsRed, as indicated on the top (scale bar: 10 μm). (B) Quantitation of mitochondrial fusion events, including kiss-and-run and complete fusion, per run ( n = 10 runs). (C) Δψm of WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. The mean TMRM FI of mitochondria in WT cells was normalized to 1. The TMRM FI per mitochondrion below 0.3 denoted low (white arrow). (D) Quantitation of percentage of mitochondria with low Δψm in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (≥20 cells for three biological replicates). (E) Quantitation of percentage of mitochondria with low Δψm in Opa1 KO cells expressing ShLuc plus Flag and Opa1 KO cells expressing OPA1-iso5-Flag plus ShLuc, Sh Mfn 1, Sh Mfn 2 or Sh Mfn 1/2 (≥20 cells for three biological replicates). ** p < 0.01, one-way ANOVA.

    Techniques Used: Labeling, Expressing, Quantitation Assay

    OPA1 Exon-4b and Exon4 partially recover mitochondrial respiration activity. (A) OCR measurements in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. (B) Relative ATP production, basal, and maximal respiration using OCR measurements in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag ( n = 2, biological replicates). WT MEF cells were used as control, whose ATP production, basal, and maximal respiration were normalized to 1. (C) Cellular ATP levels in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag, with or without oligomycin treatment ( n = 3, biological replicates). * p < 0.05, ** p < 0.01, one-way ANOVA.
    Figure Legend Snippet: OPA1 Exon-4b and Exon4 partially recover mitochondrial respiration activity. (A) OCR measurements in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. (B) Relative ATP production, basal, and maximal respiration using OCR measurements in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag ( n = 2, biological replicates). WT MEF cells were used as control, whose ATP production, basal, and maximal respiration were normalized to 1. (C) Cellular ATP levels in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag, with or without oligomycin treatment ( n = 3, biological replicates). * p < 0.05, ** p < 0.01, one-way ANOVA.

    Techniques Used: Activity Assay, Expressing, Control

    OPA1-Exon4b maintains normal TFAM distribution. (A) N-SIM Images of TFAM distribution in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. (B) Quantification of the percentage of mitochondria with diffuse TFAM in panel (A) (≥15 cells for three biological replicates, scale bar: 10 μm). (C) Anti-DNA immunofluorescence in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (scale bar: 10 μm). (D) Quantification of mtDNA nucleoid number per cell in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (≥20 cells for three biological replicates). * p < 0.05, ** p < 0.01, one-way ANOVA.
    Figure Legend Snippet: OPA1-Exon4b maintains normal TFAM distribution. (A) N-SIM Images of TFAM distribution in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. (B) Quantification of the percentage of mitochondria with diffuse TFAM in panel (A) (≥15 cells for three biological replicates, scale bar: 10 μm). (C) Anti-DNA immunofluorescence in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (scale bar: 10 μm). (D) Quantification of mtDNA nucleoid number per cell in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (≥20 cells for three biological replicates). * p < 0.05, ** p < 0.01, one-way ANOVA.

    Techniques Used: Expressing, Immunofluorescence

    OPA1-Exon4b modulates mtDNA transcription. (A,B) Anti-Flag ChIP was carried out using WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. The precipitated DNA was analyzed by qPCR using primer pairs for the D-loop region (A) or Cox1 (B) . n = 3, biological replicates. (C) Anti-Flag ChIP was carried out using WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, NT-Exon4-Flag, or NT-Exon4/4b-Flag. The precipitated DNA was analyzed by qPCR using primer pairs for the D-loop region. n = 3, biological replicates. (D) Relative mRNA levels of 9 mtDNA genes in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag ( n = 3, biological replicates). (E) Relative mRNA levels of three nuclear genes encoding respiratory subunits in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag ( n = 3, biological replicates). (F) Western blotting analysis of mtDNA-encoded Cox2 in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag. Band densities were quantified using ImageJ, and relative band densities are shown on the bottom. n = 3, biological replicates. * p < 0.05, ** p < 0.01, one-way ANOVA.
    Figure Legend Snippet: OPA1-Exon4b modulates mtDNA transcription. (A,B) Anti-Flag ChIP was carried out using WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. The precipitated DNA was analyzed by qPCR using primer pairs for the D-loop region (A) or Cox1 (B) . n = 3, biological replicates. (C) Anti-Flag ChIP was carried out using WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, NT-Exon4-Flag, or NT-Exon4/4b-Flag. The precipitated DNA was analyzed by qPCR using primer pairs for the D-loop region. n = 3, biological replicates. (D) Relative mRNA levels of 9 mtDNA genes in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag ( n = 3, biological replicates). (E) Relative mRNA levels of three nuclear genes encoding respiratory subunits in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag ( n = 3, biological replicates). (F) Western blotting analysis of mtDNA-encoded Cox2 in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag. Band densities were quantified using ImageJ, and relative band densities are shown on the bottom. n = 3, biological replicates. * p < 0.05, ** p < 0.01, one-way ANOVA.

    Techniques Used: Expressing, Western Blot



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    control wt mef cells  (ATCC)
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    ATCC control wt mef cells
    <t>OPA1-Exon4b</t> is not required for mitochondrial fusion but ensues mitochondrial bioenergetic recovery. (A) Labeling of one subset of mitochondria by photoactivation of PAGFP in cells expressing both mtPAGFP and mtDsRed, as indicated on the top (scale bar: 10 μm). (B) Quantitation of mitochondrial fusion events, including kiss-and-run and complete fusion, per run ( n = 10 runs). (C) Δψm of WT <t>MEF</t> cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. The mean TMRM FI of mitochondria in WT cells was normalized to 1. The TMRM FI per mitochondrion below 0.3 denoted low (white arrow). (D) Quantitation of percentage of mitochondria with low Δψm in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (≥20 cells for three biological replicates). (E) Quantitation of percentage of mitochondria with low Δψm in Opa1 KO cells expressing ShLuc plus Flag and Opa1 KO cells expressing OPA1-iso5-Flag plus ShLuc, Sh Mfn 1, Sh Mfn 2 or Sh Mfn 1/2 (≥20 cells for three biological replicates). ** p < 0.01, one-way ANOVA.
    Control Wt Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control wt mef cells/product/ATCC
    Average 97 stars, based on 1 article reviews
    control wt mef cells - by Bioz Stars, 2026-02
    97/100 stars
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    Image Search Results


    OPA1-Exon4b is not required for mitochondrial fusion but ensues mitochondrial bioenergetic recovery. (A) Labeling of one subset of mitochondria by photoactivation of PAGFP in cells expressing both mtPAGFP and mtDsRed, as indicated on the top (scale bar: 10 μm). (B) Quantitation of mitochondrial fusion events, including kiss-and-run and complete fusion, per run ( n = 10 runs). (C) Δψm of WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. The mean TMRM FI of mitochondria in WT cells was normalized to 1. The TMRM FI per mitochondrion below 0.3 denoted low (white arrow). (D) Quantitation of percentage of mitochondria with low Δψm in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (≥20 cells for three biological replicates). (E) Quantitation of percentage of mitochondria with low Δψm in Opa1 KO cells expressing ShLuc plus Flag and Opa1 KO cells expressing OPA1-iso5-Flag plus ShLuc, Sh Mfn 1, Sh Mfn 2 or Sh Mfn 1/2 (≥20 cells for three biological replicates). ** p < 0.01, one-way ANOVA.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: OPA1-Exon4b Binds to mtDNA D-Loop for Transcriptional and Metabolic Modulation, Independent of Mitochondrial Fusion

    doi: 10.3389/fcell.2020.00180

    Figure Lengend Snippet: OPA1-Exon4b is not required for mitochondrial fusion but ensues mitochondrial bioenergetic recovery. (A) Labeling of one subset of mitochondria by photoactivation of PAGFP in cells expressing both mtPAGFP and mtDsRed, as indicated on the top (scale bar: 10 μm). (B) Quantitation of mitochondrial fusion events, including kiss-and-run and complete fusion, per run ( n = 10 runs). (C) Δψm of WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. The mean TMRM FI of mitochondria in WT cells was normalized to 1. The TMRM FI per mitochondrion below 0.3 denoted low (white arrow). (D) Quantitation of percentage of mitochondria with low Δψm in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (≥20 cells for three biological replicates). (E) Quantitation of percentage of mitochondria with low Δψm in Opa1 KO cells expressing ShLuc plus Flag and Opa1 KO cells expressing OPA1-iso5-Flag plus ShLuc, Sh Mfn 1, Sh Mfn 2 or Sh Mfn 1/2 (≥20 cells for three biological replicates). ** p < 0.01, one-way ANOVA.

    Article Snippet: Opa1 KO and control WT MEF cells were purchased from ATCC (Manassas, United States).

    Techniques: Labeling, Expressing, Quantitation Assay

    OPA1 Exon-4b and Exon4 partially recover mitochondrial respiration activity. (A) OCR measurements in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. (B) Relative ATP production, basal, and maximal respiration using OCR measurements in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag ( n = 2, biological replicates). WT MEF cells were used as control, whose ATP production, basal, and maximal respiration were normalized to 1. (C) Cellular ATP levels in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag, with or without oligomycin treatment ( n = 3, biological replicates). * p < 0.05, ** p < 0.01, one-way ANOVA.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: OPA1-Exon4b Binds to mtDNA D-Loop for Transcriptional and Metabolic Modulation, Independent of Mitochondrial Fusion

    doi: 10.3389/fcell.2020.00180

    Figure Lengend Snippet: OPA1 Exon-4b and Exon4 partially recover mitochondrial respiration activity. (A) OCR measurements in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. (B) Relative ATP production, basal, and maximal respiration using OCR measurements in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag ( n = 2, biological replicates). WT MEF cells were used as control, whose ATP production, basal, and maximal respiration were normalized to 1. (C) Cellular ATP levels in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag, with or without oligomycin treatment ( n = 3, biological replicates). * p < 0.05, ** p < 0.01, one-way ANOVA.

    Article Snippet: Opa1 KO and control WT MEF cells were purchased from ATCC (Manassas, United States).

    Techniques: Activity Assay, Expressing, Control

    OPA1-Exon4b maintains normal TFAM distribution. (A) N-SIM Images of TFAM distribution in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. (B) Quantification of the percentage of mitochondria with diffuse TFAM in panel (A) (≥15 cells for three biological replicates, scale bar: 10 μm). (C) Anti-DNA immunofluorescence in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (scale bar: 10 μm). (D) Quantification of mtDNA nucleoid number per cell in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (≥20 cells for three biological replicates). * p < 0.05, ** p < 0.01, one-way ANOVA.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: OPA1-Exon4b Binds to mtDNA D-Loop for Transcriptional and Metabolic Modulation, Independent of Mitochondrial Fusion

    doi: 10.3389/fcell.2020.00180

    Figure Lengend Snippet: OPA1-Exon4b maintains normal TFAM distribution. (A) N-SIM Images of TFAM distribution in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. (B) Quantification of the percentage of mitochondria with diffuse TFAM in panel (A) (≥15 cells for three biological replicates, scale bar: 10 μm). (C) Anti-DNA immunofluorescence in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (scale bar: 10 μm). (D) Quantification of mtDNA nucleoid number per cell in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (≥20 cells for three biological replicates). * p < 0.05, ** p < 0.01, one-way ANOVA.

    Article Snippet: Opa1 KO and control WT MEF cells were purchased from ATCC (Manassas, United States).

    Techniques: Expressing, Immunofluorescence

    OPA1-Exon4b modulates mtDNA transcription. (A,B) Anti-Flag ChIP was carried out using WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. The precipitated DNA was analyzed by qPCR using primer pairs for the D-loop region (A) or Cox1 (B) . n = 3, biological replicates. (C) Anti-Flag ChIP was carried out using WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, NT-Exon4-Flag, or NT-Exon4/4b-Flag. The precipitated DNA was analyzed by qPCR using primer pairs for the D-loop region. n = 3, biological replicates. (D) Relative mRNA levels of 9 mtDNA genes in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag ( n = 3, biological replicates). (E) Relative mRNA levels of three nuclear genes encoding respiratory subunits in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag ( n = 3, biological replicates). (F) Western blotting analysis of mtDNA-encoded Cox2 in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag. Band densities were quantified using ImageJ, and relative band densities are shown on the bottom. n = 3, biological replicates. * p < 0.05, ** p < 0.01, one-way ANOVA.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: OPA1-Exon4b Binds to mtDNA D-Loop for Transcriptional and Metabolic Modulation, Independent of Mitochondrial Fusion

    doi: 10.3389/fcell.2020.00180

    Figure Lengend Snippet: OPA1-Exon4b modulates mtDNA transcription. (A,B) Anti-Flag ChIP was carried out using WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. The precipitated DNA was analyzed by qPCR using primer pairs for the D-loop region (A) or Cox1 (B) . n = 3, biological replicates. (C) Anti-Flag ChIP was carried out using WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, NT-Exon4-Flag, or NT-Exon4/4b-Flag. The precipitated DNA was analyzed by qPCR using primer pairs for the D-loop region. n = 3, biological replicates. (D) Relative mRNA levels of 9 mtDNA genes in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag ( n = 3, biological replicates). (E) Relative mRNA levels of three nuclear genes encoding respiratory subunits in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag ( n = 3, biological replicates). (F) Western blotting analysis of mtDNA-encoded Cox2 in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag. Band densities were quantified using ImageJ, and relative band densities are shown on the bottom. n = 3, biological replicates. * p < 0.05, ** p < 0.01, one-way ANOVA.

    Article Snippet: Opa1 KO and control WT MEF cells were purchased from ATCC (Manassas, United States).

    Techniques: Expressing, Western Blot